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Prevalence and Characterization of Cronobacter spp. from Various Foods, Medicinal Plants, and Environmental Samples

Author:
Singh, Niharika, Goel, Gunjan, Raghav, Mamta
Source:
Current microbiology 2015 v.71 no.1 pp. 31-38
ISSN:
0343-8651
Subject:
Cronobacter sakazakii, aminoglycosides, antibiotic resistance, bacterial contamination, beta-lactams, food contamination, food matrix, genes, herbs, macrolides, medicinal plants, milk substitutes, pathogens, screening, spices, tetracyclines
Abstract:
Dairy or non-dairy based products were explored to determine the prevalence, molecular characterization, and antibiotic susceptibility of Cronobacter spp. The isolation was done as per ISO 22964:2006 on chromogenic media followed by further confirmation by biochemical- and 16S rRNA-based identification. From 219 samples, the chromogenic agar assay and biochemical tests yielded presumptive 45 isolates. Among them, only 36 isolates showed 282 bp band amplified from ITS-G gene confirming as Cronobacter sakazakii. The Cronobacter spp. prevalence was highest in herbs and spices (34 %) while environmental samples had contamination rates of 23 % indicating plants as a possible reservoir of this pathogen. All the isolates were resistant to β-lactam derivatives (68 %), macrolides (88.6 %), and aminoglycosides (79.9 %) but susceptible to phenicoles (31.6 %) and tetracyclines (15 %) derivatives. The results emphasize the screening of plant materials before their incorporation in food matrices.
Agid:
3406860