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5‐Caffeoylquinic acid decreases diet‐induced obesity in rats by modulating PPARα and LXRα transcription

Huang, Kang, Liang, Xiu‐ci, Zhong, Ying‐li, He, Wan‐yan, Wang, Zheng
Journal of the science of food and agriculture 2015 v.95 no.9 pp. 1903-1910
beta oxidation, beverages, blood lipids, blood serum, chlorogenic acid, cholesterol, dose response, droplets, fatty acids, free fatty acids, gene expression, genes, hepatocytes, high fat diet, lipid metabolism disorders, liver, messenger RNA, obesity, quantitative polymerase chain reaction, rats, signal transduction, transcription factors, vacuoles
BACKGROUND: Chlorogenic acids (CGAs) are widely distributed in plant material, including foods and beverages. 5‐Caffeoylquinic acid (5‐CQA) is the most studied CGA, but the mechanism of its hypolipidaemic effect remains unclear. This study aimed to determine the effect of 5‐CQA on lipid metabolism in the liver of Sprague–Dawley rats fed a high‐fat diet (HFD). RESULTS: 5‐CQA suppressed HFD‐induced increases in body weight and visceral fat‐pad weight, serum lipid levels, and serum and hepatic free fatty acids in a dose‐dependent manner. Real‐time polymerase chain reaction revealed that 5‐CQA altered the mRNA expression of the transcription factors peroxisome proliferator‐activated receptor α (PPARα) and liver X receptor α (LXRα) and target genes involved in hepatic fatty acid uptake, β‐oxidation, fatty acid synthesis, and cholesterol synthesis. Moreover, hepatic tissue sections from HFD‐fed rats showed many empty vacuoles, suggesting that liver cells were filled with more fat droplets. However, 5‐CQA significantly ameliorated this effect. CONCLUSION: 5‐CQA may improve lipid metabolism disorders by altering the expression of PPARα and LXRα, which are involved in multiple intracellular signalling pathways. © 2014 Society of Chemical Industry