PubAg

Main content area

Fine mapping of qSS‐9, a major and stable quantitative trait locus, for seed storability in rice (Oryza sativa L.)

Author:
Lin, Qiuyun, Jiang, Yimei, Sun, Ailing, Cao, Penghui, Li, Linfang, Liu, Xi, Tian, Yunlu, He, Jun, Liu, Shijia, Chen, Liangming, Jiang, Ling, Ahn, S.N.
Source:
Plant breeding 2015 v.134 no.3 pp. 293-299
ISSN:
0179-9541
Subject:
Oryza sativa, agronomic traits, alleles, backcrossing, chromosome mapping, genetic background, isogenic lines, loci, molecular cloning, progeny, quantitative trait loci, rice, storage quality, substitution lines
Abstract:
Seed storability in rice (Oryza sativa L.) is an important agronomic trait. We previously showed a quantitative trait locus of seed storability, qSS‐9, on chromosome 9 in a backcross population of ‘Koshihikari’ (japonica) / ‘Kasalath’ (indica) // ‘Koshihikari’. In this study, fine mapping of the chromosomal location of qSS‐9 was performed. Effect of ‘Kasalath’ allele of qSS‐9 was validated using a chromosome segment substitution line, SL36, which harboured the target quantitative trait loci (QTL) from ‘Kasalath’ in the genetic background of ‘Nipponbare’ under different ageing treatments in different environments. Subsequently, an F₂ population from a cross between ‘Nipponbare’ and SL36 was used for fine mapping of qSS‐9. Simultaneously, four subnear isogenic lines (sub‐NILs) that represented different recombination breakpoints across the qSS‐9 region were developed from F₃ progeny. Finally, the qSS‐9 locus was located between the Indel markers Y10 and Y13, which delimit a region of 147 kb in the ‘Nipponbare’ genome. These results provide a springboard for map‐based cloning of qSS‐9 and possibilities for breeding rice varieties with strong seed storability.
Agid:
3408625