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Comparative Genomics of the IncA/C Multidrug Resistance Plasmid Family

Author:
Fricke, W. Florian, Welch, Timothy J., McDermott, Patrick F., Mammel, Mark K., LeClerc, J. Eugene, White, David G., Cebula, Thomas A., Ravel, Jacques
Source:
Journal of bacteriology 2009 v.191 no.15 pp. 4750
ISSN:
1098-5530
Subject:
Aeromonas hydrophila, animal pathogenic bacteria, fish diseases, Escherichia coli, plasmids, nucleotide sequences, genomics, molecular genetics, multiple drug resistance, evolution, operon, conjugation
Abstract:
Multidrug resistance (MDR) plasmids belonging to the IncA/C plasmid family are widely distributed among Salmonella and other enterobacterial isolates from agricultural sources and have, at least once, also been identified in a drug-resistant Yersinia pestis isolate (IP275) from Madagascar. Here, we present the complete plasmid sequences of the IncA/C reference plasmid pRA1 (143,963 bp), isolated in 1971 from the fish pathogen Aeromonas hydrophila, and of the cryptic IncA/C plasmid pRAx (49,763 bp), isolated from Escherichia coli transconjugant D7-3, which was obtained through pRA1 transfer in 1980. Using comparative sequence analysis of pRA1 and pRAx with recent members of the IncA/C plasmid family, we show that both plasmids provide novel insights into the evolution of the IncA/C MDR plasmid family and the minimal machinery necessary for stable IncA/C plasmid maintenance. Our results indicate that recent members of the IncA/C plasmid family evolved from a common ancestor, similar in composition to pRA1, through stepwise integration of horizontally acquired resistance gene arrays into a conserved plasmid backbone. Phylogenetic comparisons predict type IV secretion-like conjugative transfer operons encoded on the shared plasmid backbones to be closely related to a group of integrating conjugative elements, which use conjugative transfer for horizontal propagation but stably integrate into the host chromosome during vegetative growth. A hipAB toxin-antitoxin gene cluster found on pRA1, which in Escherichia coli is involved in the formation of persister cell subpopulations, suggests persistence as an early broad-spectrum antimicrobial resistance mechanism in the evolution of IncA/C resistance plasmids.
Agid:
36243
Handle:
10113/36243