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Liquid Chromatography–Hybrid Linear Ion Trap–High-Resolution Mass Spectrometry (LTQ-Orbitrap) Method for the Determination of Glycoalkaloids and Their Aglycons in Potato Samples

Caprioli, Giovanni, Cahill, Michael G., Vittori, Sauro, James, Kevin J.
Food analytical methods 2014 v.7 no.7 pp. 1367-1372
alpha-chaconine, cooking, diet, ions, mass spectrometry, methanol, monitoring, potatoes, secondary metabolites, solanine
Potatoes produce biologically active secondary metabolites like glycoalkaloids and their aglycons, which may have both adverse and beneficial effects in the diet. A new analytical method that uses liquid chromatography–mass spectrometry (LTQ-Orbitrap) has been developed for the analysis of glycoalkaloids and their aglycons in potato samples. Two glycoalkaloids, α-solanine and α-chaconine, and two aglycons, demissidine and solasodine, were quantified in potato samples. Samples were extracted using methanol, purified on an SPE Strata C₁₈ cartridge, and then analyzed in HPLC–mass spectrometry (LTQ-Orbitrap) with the FTMS operating in full scan at a resolving power of 30,000 (FWHM), enabling the detection and accurate mass measurement and with the ITMS mode operating in MRM (multiple reaction monitoring) for glycoalkaloids and their aglycons using the [M + H]⁺ ions and their optimized collision energies. After validation, the method was applied to screen different type of potatoes, and some cooking experiment were conducted.