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A Rapid Quantification of β-Carotene in Fruits and Vegetables by Dispersive Liquid–Liquid Microextraction Coupled with UV–Vis Spectrophotometry: Optimized by Response Surface Methodology

Author:
Sereshti, Hassan, Ahmadvand, Mohammad, Asgari, Shohreh
Source:
Food analytical methods 2014 v.7 no.7 pp. 1481-1488
ISSN:
1936-9751
Subject:
beta-carotene, chloroform, detection limit, fruits, liquid-liquid extraction, methanol, microextraction, response surface methodology, salt concentration, saponification, solvents, vegetables
Abstract:
A simple, fast, and efficient method consisted of optimized dispersive liquid–liquid microextraction (DLLME) followed by UV–vis spectrophotometry was developed for determination of β-carotene in fruits and vegetables. Chloroform and methanol were chosen as extraction and disperser solvents, respectively. The extraction process was optimized using a central composite design (CCD) with the optimum points of 115 μL for volume of extraction solvent and 6.5 % (w/v) for salt concentration. Under the optimal conditions, the relative standard deviation (RSD, C = 500 μg L⁻¹, n = 5), limit of detection (LOD), linear dynamic range (LDR), and coefficient of determination (R²) were 1.08 %, 2 μg L⁻¹, 50–1,500 μg L⁻¹, and 0.991, respectively. The present method consisted of a simple and fast sample preparation procedure without any antioxidant addition, saponification, and purification was used.
Agid:
362891