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Compositions and kits for detecting a sequence mutation in cinnamyl alcohol dehydrogenase gene associated with altered lignification in loblolly pine

Gill, Geoffrey P., Brown, Garth R., Neale, David B.
United States Department of Agriculture patents 2008 no.US 7,396,921 B2
Pinus taeda, cinnamyl alcohol dehydrogenase, mutation, alleles, lignification, single nucleotide polymorphism, genetic techniques and protocols, patents, USDA, United States
Loblolly pine (Pinus taeda L.) is the most important commercial tree species in the USA harvested for pulp and solid wood products. Increasing the efficiency of chemical pulping may be achieved through the manipulation of genes involved in the lignin biosynthetic pathway. A null allele of cinnamyl alcohol dehydrogenase (CAD) has been discovered in the loblolly pine clone 7-56 which displays altered lignin composition. During identification of single nucleotide polymorphisms (SNPs) in the cad gene, a two-base pair adenosine insertion located in exon five and unique to clone 7-56 was discovered. The sequence mutation causes a frame-shift predicted to result in premature termination of the protein. For routine detection of the mutation, a diagnostic assay was developed utilising Template-directed Dye-terminator Incorporation and Fluorescence Polarization detection (FP-TDI).