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Production of enzyme preparations on the basis of Penicillum canescens recombinant strains with a high ability for the hydrolysis of plant materials

Author:
Volkov, P. V., Rozhkova, A. M., Pravilnikov, A. G., Andrianov, R. M., Dotsenko, G. S., Bekkarevich, A. O., Koshelev, A. V., Okunev, O. N., Zorov, I. N., Sinitsin, A. P.
Source:
Applied biochemistry and microbiology 2012 v.48 no.1 pp. 58-64
ISSN:
0003-6838
Subject:
Talaromyces verruculosus, Aspergillus niger, xylanases, wood, wood pulp, Penicillium, pulping, hydrolysis, beta-glucosidase, fungi
Abstract:
An enzyme preparation has been produced on the basis of Penicillium canescens strains with the activity of cellibiohydrolase I, II; endo-1,4-β-gluconase of Penicillium verruculosum; and β-glucosidase of Aspergillus niger. It was shown that for the most effective hydrolysis of aspen wood pulp the optimal ratio of cellobiohydrolase and endo-1,4-β-gluconase in enzyme preparations was 8: 2 (by protein). It was also established that the homologous xylanase secreted by the Penicillium canescens fungus is a required component for the enzyme complex for hydrolysis of the hemicellulose matrix of aspen wood.
Agid:
371883