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Identification and Characterization of Two Distinct Bursal B-Cell Subpopulations Following Infectious Bursal Disease Virus Infection of White Leghorn Chickens
- Petkov, Daniel I., Linnemann, Erich G., Kapczynski, Darrell R., Sellers, Holly S.
- Avian diseases 2009 v.53 no.3 pp. 347
- chickens, White Leghorn, bursa of Fabricius, infectious bursal disease, Infectious bursal disease virus, vertebrate viruses, immunoglobulin M, B-lymphocytes, phenotype, spleen, signs and symptoms (animals and humans), major histocompatibility complex, viral antigens, vaccination, temporal variation, immunoglobulin A, immunoglobulin G, gene expression
- Infectious bursal disease virus (IBDV) is an immunosuppressive virus which primarily infects IgM B-cells in the bursa of Fabricius. Flow cytometric analysis was used to phenotype B-cell populations in the bursa and spleen following IBDV infection. In the bursa, two IgM B-cell subpopulations, designated as A and B, were identified based on cell size and granularity. While both subpopulations differentially expressed IgM and Bu-1b surface markers, both groups displayed major histocompatibility complex class II surface antigens at equal levels. Following IBDV challenge of nonvaccinated birds, the B subpopulation was significantly reduced between 7 and 21 days postchallenge compared to either nonchallenged birds or vaccinated-challenged birds. However, the reduction of subpopulation B in the bursa, following IBDV exposure, did not reduce the levels of total serum IgA, IgG, and IgM, nor did it affect IgG and IgA B-cells in the spleen. Phenotypic analysis of the subpopulations identified differential expression of Lewis(x), IgM, Bu-1b, and MUI78 surface antigens between the subpopulations. Overall, these are the first studies to identify two distinct IgM B-cell subpopulations in the chicken bursa, and the first to describe the decrease in the IgM B-cell population relative to IgA and IgG B-cells following IBDV infection.