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Differential Expression of Durum Wheat Gluten Proteome under Water Stress during Grain Filling

Giuliani, Marcella Michela, Palermo, Carmen, De Santis, Michele Andrea, Mentana, Annalisa, Pompa, Marianna, Giuzio, Luigia, Masci, Stefania, Centonze, Diego, Flagella, Zina
Journal of agricultural and food chemistry 2015 v.63 no.29 pp. 6501-6512
Triticum turgidum subsp. durum, cultivars, durum wheat, filling period, gene expression regulation, gliadin, glutenins, protein composition, proteome, proteomics, seed development, water stress, wheat gluten, wheat protein
Environmental stress during grain filling may affect wheat protein composition, thus influencing its final quality. A proteomic approach was used to evaluate changes in storage protein composition under water stress of two Italian durum wheat (Triticum turgidum ssp. durum) cultivars, Ciccio and Svevo. The high-molecular-weight glutenin region increased progressively in both cultivars and under two water regimens. The L48–35 region, corresponding to low-molecular-weight (LMW) glutenin subunits, increased slightly during grain development and decreased under water stress in both cultivars. In particular, an s-type LMW related to superior technological quality was down-expressed in the early–mid period in Svevo and in the mid–late period in Ciccio. Finally, the L<35 region, corresponding to gliadin-like proteins, decreased slightly during grain development and increased under stress in both cultivars. Several α-gliadins, associated with immunological potential, increased their expression under water stress, especially in Svevo in the early–mid stage of grain filling.