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Differential Expression of Durum Wheat Gluten Proteome under Water Stress during Grain Filling

Author:
Giuliani, Marcella Michela, Palermo, Carmen, De Santis, Michele Andrea, Mentana, Annalisa, Pompa, Marianna, Giuzio, Luigia, Masci, Stefania, Centonze, Diego, Flagella, Zina
Source:
Journal of agricultural and food chemistry 2015 v.63 no.29 pp. 6501-6512
ISSN:
0021-8561
Subject:
Triticum turgidum subsp. durum, cultivars, durum wheat, filling period, gene expression regulation, gliadin, glutenins, protein composition, proteome, proteomics, seed development, water stress, wheat gluten, wheat protein
Abstract:
Environmental stress during grain filling may affect wheat protein composition, thus influencing its final quality. A proteomic approach was used to evaluate changes in storage protein composition under water stress of two Italian durum wheat (Triticum turgidum ssp. durum) cultivars, Ciccio and Svevo. The high-molecular-weight glutenin region increased progressively in both cultivars and under two water regimens. The L48–35 region, corresponding to low-molecular-weight (LMW) glutenin subunits, increased slightly during grain development and decreased under water stress in both cultivars. In particular, an s-type LMW related to superior technological quality was down-expressed in the early–mid period in Svevo and in the mid–late period in Ciccio. Finally, the L<35 region, corresponding to gliadin-like proteins, decreased slightly during grain development and increased under stress in both cultivars. Several α-gliadins, associated with immunological potential, increased their expression under water stress, especially in Svevo in the early–mid stage of grain filling.
Agid:
3729728