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Volumetric measurement of human red blood cells by MOSFET‐based microfluidic gate

Guo, Jinhong, Ai, Ye, Cheng, Yuanbing, Li, Chang Ming, Kang, Yuejun, Wang, Zhiming
Electrophoresis 2015 v.36 no.16 pp. 1862-1865
adults, bioassays, electrophoresis, erythrocytes, humans, monitoring, neonates, polystyrenes, semiconductors
In this paper, we present a MOSFET‐based (metal oxide semiconductor field‐effect transistor) microfluidic gate to characterize the translocation of red blood cells (RBCs) through a gate. In the microfluidic system, the bias voltage modulated by the particles or biological cells is connected to the gate of MOSFET. The particles or cells can be detected by monitoring the MOSFET drain current instead of DC/AC‐gating method across the electronic gate. Polystyrene particles with various standard sizes are utilized to calibrate the proposed device. Furthermore, RBCs from both adults and newborn blood sample are used to characterize the performance of the device in distinguishing the two types of RBCs. As compared to conventional DC/AC current modulation method, the proposed device demonstrates a higher sensitivity and is capable of being a promising platform for bioassay analysis.