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Isolation and purification of prenylated phenolics from Amorpha fruticosa by high‐speed counter‐current chromatography
- Chen, Chu, Wu, Yan, Chen, Yang, Du, Leilei
- Journal of separation science 2015 v.38 no.16 pp. 2924-2929
- Amorpha fruticosa, anti-inflammatory activity, countercurrent chromatography, fruits, mass spectrometry, nuclear magnetic resonance spectroscopy, phenolic compounds, solvents, ultra-performance liquid chromatography
- Prenylated phenolics such as amorfrutins are recently identified potent anti‐inflammatory and antidiabetic natural products. In this work, high‐speed counter‐current chromatography was investigated for the isolation and purification of prenylated phenolics from the fruits of Amorpha fruticosa by using a two‐phase solvent system composed of n‐hexane/ethanol/water (5:4:1, v/v). As a result, 14.2 mg of 5,7‐dihydroxy‐8‐geranylflavanone, 10.7 mg of amorfrutin A and 17.4 mg of amorfrutin B were obtained from 200 mg of n‐hexane‐soluble crude extract in one step within 250 min. The purities of 5,7‐dihydroxy‐8‐geranylflavanone, amorfrutins A and B were 95.2, 96.7 and 97.1%, respectively, as determined by ultra high performance liquid chromatography. The structural identification was performed by mass spectrometry and ¹H and ¹³C NMR spectroscopy. The results indicated that the established method is an efficient and convenient way to purified prenylated phenolics from A. fruticosa extract.