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Hydrogen Sulfide Alleviates Postharvest Senescence of Broccoli by Modulating Antioxidant Defense and Senescence-Related Gene Expression

Author:
Li, Shi-Ping, Hu, Kang-Di, Hu, Lan-Ying, Li, Yan-Hong, Jiang, An-Min, Xiao, Fang, Han, Yi, Liu, Yong-Sheng, Zhang, Hua
Source:
Journal of agricultural and food chemistry 2014 v.62 no.5 pp. 1119-1129
ISSN:
0021-8561
Subject:
ascorbate peroxidase, genes, metabolites, dose response, phenylalanine ammonia-lyase, malondialdehyde, hydrogen peroxide, gene expression, carotenoids, superoxide anion, hydrogen sulfide, gene expression regulation, peroxidase, cysteine proteinases, anthocyanins, broccoli, catechol oxidase, linoleate 13S-lipoxygenase, fruits, glutathione-disulfide reductase, catalase, chlorophyll
Abstract:
Accumulating evidence has shown that hydrogen sulfide (H2S) acts as a signaling regulator in plants. Here we show that H2S delays the postharvest senescence of broccoli in a dose-dependent manner. H2S maintains higher levels of metabolites, such as carotenoids, anthocyanin, and ascorbate, and reduces the accumulation of malondialdehyde, H2O2, and the superoxide anion. Further investigations showed that H2S sustained higher activities of guaiacol peroxidase, ascorbate peroxidase, catalase, and glutathione reductase and lower activities of lipoxygenase, polyphenol oxidase, phenylalanine ammonia lyase, and protease than those of water control. Moreover, the expression of the chlorophyll degradation related genes BoSGR, BoCLH2, BoPaO, BoRCCR, as well as cysteine protease BoCP1 and lipoxygenase gene BoLOX1, was down-regulated in postharvest broccoli treated with H2S. The functions of H2S on the senescence of other vegetables and fruits suggest its universal role acting as a senescence regulator.
Agid:
3762546