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Effect of various factors on the biosynthesis of piscarinines, secondary metabolites of the fungus Penicillium piscarium Westling

Author:
Zhelifonova, V. P., Maier, A., Kozlovskii, A. G.
Source:
Applied biochemistry and microbiology 2008 v.44 no.6 pp. 608-612
ISSN:
0003-6838
Subject:
Penicillium, biosynthesis, conidia, culture media, fungi, glucose, inhibitory concentration 50, ions, iron, mannitol, prostatic neoplasms, secondary metabolites, zinc
Abstract:
Piscarinines A and B were synthesized most actively during the surface cultivation of the fungus Penicillium piscarium in a complex medium (5.5 mg/l). Under conditions of submerged cultivation in a mineral medium, the yield of piscarinines was two times lower. An increase in the inoculum quantity of conidia treated with Tween-80 increased the culture productivity. The biosynthesis of the alkaloid was completely suppressed when mannitol was replaced with glucose or when zinc, iron, or copper ions were added to the culture medium. The metabolites were active against the prostate cancer cell line LNCAP (IC₅₀ were 2.195 and 1.914 μg/ml for piscarinines A and B, respectively).
Agid:
378622