PubAg

Main content area

Development of orthogonal two-dimensional hydrophilic interaction chromatography systems with the introduction of novel stationary phases

Author:
Liu, Yanming, Guo, Zhimou, Feng, Jiatao, Xue, Xingya, Zhang, Feifang, Xu, Qing, Liang, Xinmiao
Source:
Journal of separation science 2009 v.32 no.17 pp. 2871-2876
ISSN:
1615-9306
Subject:
Carthamus tinctorius, Oriental traditional medicine, chromatography, hydrophilic interactions, particle size
Abstract:
Two different offline 2-D hydrophilic interaction chromatography (2D-HILIC/HILIC) systems have been developed. In the two systems, a click maltose column was used in the first dimension, an amide column or a click β-CD column was used in the second dimension, respectively. Both of the systems were used for the analysis of very polar components in Carthamus tinctorius Linn., which is a traditional Chinese medicine. Excellent orthogonality and separation results were obtained in both 2D-HILIC/HILIC systems, while the peak capacity of the system based on click maltose and amide column was higher for its adoption of stationary phase with smaller particle size in the second dimension.
Agid:
379720