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Quantification of D-Asp and D-Glu in rat brain and human cerebrospinal fluid by microchip electrophoresis
- Huang, Yong, Shi, Ming, Zhao, Shulin
- Journal of separation science 2009 v.32 no.17 pp. 3001-3006
- brain, cerebrospinal fluid, derivatization, electrophoresis, glass, glutamic acid, humans, rats
- A microchip electrophoresis (MCE) method with LIF detection was presented for quantification of D-aspartic acid (D-Asp) and D-glutamate (D-Glu) in biological samples. D-Asp and D-Glu were determined after precolumn derivatization with FITC. The chiral separation was performed on a glass/PDMS hybrid microfluidic chip using γ-CD as chiral selector in the running buffer. High sensitive detection was obtained by the LIF detection. The LODs (S/N = 3) for D-Asp and D-Glu were 6.0x10⁻⁸ and 4.0x10⁻⁸ M, respectively. Using this method, the levels of D-Asp and D-Glu in rat brain and human cerebrospinal fluid (CSF) were determined.