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KCNQ1 loss-of-function mutation impairs gastric acid secretion in mice

Author:
Pan, Qin, Ma, Jun, Zhou, Qinshu, Li, Jun, Tang, Yongqing, Liu, Yi, Yang, Yiqing, Xiao, Junjie, Peng, Luying, Li, Pengjuan, Liang, Dandan, Zhang, Hong, Chen, Yi-Han
Source:
Molecular biology reports 2010 v.37 no.3 pp. 1329-1333
ISSN:
0301-4851
Subject:
acidification, gastric acid, gastric mucosa, histamine, mice, mutation, protons, secretion
Abstract:
The KCNQ1 channel is abundantly expressed in the gastric parietal cells. Although the functional coupling of KCNQ1 with the H⁺/K⁺-ATPase has already been confirmed on the basis of pharmacological kinetics, the effect of a KCNQ1 loss-of-function mutation on gastric acidification remains unclear. In this study, parietal cells and gastric glands from both C57BL/6 J mice (normal control) and J343 mice (mice with a KCNQ1 loss-of-function mutation) were isolated to study the effects of KCNQ1 on gastric acidification. We found that the mutation limited intracellular acidification of parietal cells and H⁺ secretion of the stomach in response to histamine. Thus, a KCNQ1 loss-of-function mutation may impair gastric acid secretion.
Agid:
380232