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Plants as Biofactories: Physiological Role of Reactive Oxygen Species on the Accumulation of Phenolic Antioxidants in Carrot Tissue under Wounding and Hyperoxia Stress

Author:
Jacobo-Velazquez, Daniel A., Martínez-Hernández, Ginés B., Rodríguez, Silvia del C., Cao, Cong-Mei, Cisneros-Zevallos, Luis
Source:
Journal of agricultural and food chemistry 2011 v.59 no.12 pp. 6583-6593
ISSN:
0021-8561
Subject:
Daucus carota, NADP (coenzyme), abiotic stress, acids, antioxidants, ascorbate peroxidase, carrots, catalase, enzyme activity, hyperoxia, phenylalanine ammonia-lyase, reactive oxygen species, secondary metabolites, superoxide dismutase
Abstract:
Plants subjected to postharvest abiotic stresses synthesize secondary metabolites with health-promoting properties. Here, we report the potential use of carrots (Daucus carota) as biofactories of caffeoylquinic acids when subjected to wounding and hyperoxia stresses. Wounding stress induced an increase of 287% in total phenolic content (PC) in carrots stored for 48 h at 20 °C. This increase was higher (349%) in the wounded tissue treated with hyperoxia stress. To further understand the physiological role of reactive oxygen species (ROS) as a signaling molecule for the stress-induced accumulation of phenolics in carrots, the respiration rate as well as the enzymatic activities of NADPH oxidase, superoxide dismutase, ascorbate peroxidase, and catalase were evaluated. Likewise, shredded carrots were treated with diphenyleneiodonium chloride solution to block NADPH oxidase ROS productions, and the phenylalanine ammonia lyase activity and total PC were evaluated. Results demonstrated that ROS play a key role as a signaling molecule for the stress-induced accumulation of PC in carrots.
Agid:
383444