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Qualitative and Quantitative Analysis of Steroidal Saponins in Crude Extract and Bark Powder of Yucca schidigera Roezl

Kowalczyk, Mariusz, Pecio, Łukasz, Stochmal, Anna, Oleszek, Wiesław
Journal of agricultural and food chemistry 2011 v.59 no.15 pp. 8058-8064
Yucca schidigera, bark, high performance liquid chromatography, ion exchange chromatography, markets, mass spectrometry, quantitative analysis, steroid saponins, syrups
Steroidal saponins in commercial stem syrup and in extract of a bark of Yucca schidigera were identified with high-performance liquid chromatography ion trap mass spectrometry and quantitated using ultraperformance liquid chromatography with quadrupole mass spectrometric detection. Fragmentation patterns of yucca saponins were generated using collision-induced dissociation and compared with fragmentation of authentic standards as well as with published spectrometric information. In addition to detection of twelve saponins known to occur in Y. schidigera, collected fragmentation data led to tentative identifications of seven new saponins. A quantitation method for all 19 detected compounds was developed and validated. Samples derived from the syrup and the bark of yucca were quantitatively measured and compared. Obtained results indicate that yucca bark accumulates polar, bidesmosidic saponins, while in the stem steroidal glycosides with middle- and short-length saccharide chains are predominant. The newly developed method provides an opportunity to evaluate the composition of yucca products available on the market.