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Melamine-Induced Urolithiasis in a Drosophila Model

Chen, Wen-Chi, Lin, Wei-Yong, Chen, Huey-Yi, Chang, Chiao-Hui, Tsai, Fuu-Jen, Man, Kee-Ming, Shen, Jui-Lung, Chen, Yung-Hsiang
Journal of agricultural and food chemistry 2012 v.60 no.10 pp. 2753-2757
Drosophila, Malpighian tubules, calcium oxalate, citrates, crystals, foods, humans, ingestion, longevity, melamine, models, polarized light, potassium
Melamine-tainted food can induce renal stones in both humans and animals. We have previously reported a novel Drosophila model for the study of renal stone disease. In addition to hyperoxaluria-causing agents, we also tested herein the effect of melamine on crystal formation in Drosophila. The results indicate that administration of melamine alone caused crystal formation in a dose-dependent manner. The crystals also appeared after ingestion of melamine for 3 weeks in the Malpighian tubules of Drosophila when viewed with polarized light. Administration of potassium citrate (K citrate) was found to significantly ameliorate the melamine-induced reduction of lifespan. However, administration of K citrate failed to reduce the quantity of crystals. Because calcium oxalate is not the major crystal induced by melamine, the predominant components of melamine-induced crystals and the potential crystal inhibitors warrant further investigation.