Jump to Main Content
Production and characterisation of enzymatically produced lauric acid esters of fructose
- Sekeroglu, Gulten, Fadiloglu, Sibel, Ibanoglu, Esra
- Journal of the science of food and agriculture 2002 v.82 no.13 pp. 1516-1522
- dodecanoic acid, esters, fructose, high performance liquid chromatography, surface tension, triacylglycerol lipase
- Lauric acid esters of fructose were produced using immobilised lipase (EC 184.108.40.206; Novozym 435). A molecular sieve was used to shift the reaction towards the synthesis. The mono-, di- and higher esters of lauric acid with fructose were obtained and analysed qualitatively and quantitatively by high-pressure liquid chromatography (HPLC). At a 5:1 molar ratio of lauric acid to fructose the amounts of monoester, diester and higher esters produced were 37, 53 and 98 mg respectively after 96 h of reaction time. After purification of the products by column chromatography the hydrophile/lipophile balance (HLB) values of fructose monolaurate and fructose dilaurate were found to be 8.95 and 5.3 respectively and their critical micelle concentration (CMC) values were calculated as 7.20 × 10−5 and 6.40 × 10−5 M respectively. The CMC values of esters obtained from the conductivity curve and the surface tension curve were significantly (P < 0.05) similar to each other. It was found that when the concentration of surfactant was increased from 0.25 to 0.50% (w/v), stabilisation of the emulsion also increased. Fructose dilaurate showed better stability than the other esters. Separation of the phases reached 70% within 30 h for the emulsion prepared with 0.50% (w/v) of this ester.