Main content area

The diurnal pattern of ileal dry matter and endogenous ileal nitrogen flows in the growing pig

Hodgkinson, Suzanne M., Moughan, Paul J., Morel, Patrick C.H., Reynolds, Gordon W.
Journal of the science of food and agriculture 2002 v.82 no.15 pp. 1860-1866
body weight, cannulas, casein, centrifugation, chromic oxide, chromium, diet, digesta, digestive tract, diurnal variation, ileum, molecular weight, nitrogen, swine, swine feeding, ultrafiltration
The aim was to examine the diurnal pattern of endogenous nitrogen flow at the terminal ileum of the pig. Seven entire male pigs with a mean body weight of 58 kg had post-valve T-caecum (PVTC) cannulae surgically implanted for the collection of ileal digesta. The pigs were fed equal-sized meals (10% of metabolic body weight, W0.75, per day) twice daily at 08:00 and 17:00. The semi-synthetic diet included enzyme-hydrolysed casein (<5000 Da, 100 g kg−1 diet) as the sole source of nitrogen (N). The diet also contained chromic oxide (6 g kg−1) as an indigestible marker. The pigs received the diet for an 8 day period, and digesta were continuously collected for 24 h periods (commencing at 08:00) on the fifth and eighth days. Digesta were analysed for dry matter (DM) and chromium (Cr), and endogenous N was determined in the precipitate + retentate fraction of the digesta following centrifugation and ultrafiltration (10 000 Da molecular weight cut-off). The concentration of Cr expressed on a digesta DM basis and the ratio of endogenous N to Cr in the digesta were relatively constant over the 24 h digesta collection periods, with no statistically significant (P > 0.05) differences found for the latter measure in the digesta collected between 12:00 and 08:00. The endogenous N flow through the terminal ileum is the net effect of the secretion and reabsorption of endogenous N that occurs throughout the digestive tract. The net result of the latter two processes in the small intestine of the pig leads to a relatively constant endogenous N flow over time post-feeding.