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Determination of PCBs in Fish Using Enzyme-linked Immunosorbent Assay (ELISA)
- Lasrado, J.A., Santerre, C.R., Zajicek, J.L., Stahl, J.R., Tillitt, D.E., Deardorff, D.
- Journal of food science 2003 v.68 no.1 pp. 133-136
- catfish, enzyme-linked immunosorbent assay, polychlorinated biphenyls, wild fish
- Polychlorinated biphenyls (PCBs) were determined in fish tissue using an enzyme-linked immunosorbent assay (ELISA). Standard curves for Aroclor 1248, 1254, and 1260 in catfish tissue were developed with ranges from 0.05 to 0.5 ppm and 0.5 to 5.0 ppm. Wild fish were initially analyzed using gas chromatography/electron-capture detection (GC/ECD) and those having residues within the standard curve ranges were analyzed with ELISA. Results obtained using ELISA and GC/ECD were not significantly different (p < 0.05) from 0.05 to 0.5 ppm. From 0.5 to 5.0 ppm, the standard curve for Aroclor 1254 was the best predictor of total PCB in wild fish samples.