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Assessment of the swine protein-annotated oligonucleotide microarray

Author:
Steibel, J.P., Wysocki, M., Lunney, J.K., Ramos, A.M., Hu, Z.-L., Rothschild, M.F., Ernst, C.W.
Source:
Animal genetics 2009 v.40 no.6 pp. 883
ISSN:
0268-9146
Subject:
swine, oligonucleotides, microarray technology, gene expression, messenger RNA, animal proteins, liver, brain, uterus, endothelium, skeletal muscle, longissimus dorsi, polymerase chain reaction, antisense RNA
Abstract:
The specificity and utility of the swine protein-annotated oligonucleotide microarray, or Pigoligoarray ( http://www.pigoligoarray.org), has been evaluated by profiling the expression of transcripts from four porcine tissues. Tools for comparative analyses of expression on the Pigoligoarray were developed including HGNC identities and comparative mapping alignments with human orthologs. Hybridization results based on the Pigoligoarray's sets of control, perfect match (PM) and deliberate mismatch (MM) probes provide an important means of assessing non-specific hybridization. Simple descriptive diagnostic analyses of PM/MM probe sets are introduced in this paper as useful tools for detecting non-specific hybridization. Samples of RNA from liver, brain stem, longissimus dorsi muscle and uterine endothelium from four pigs were prepared and hybridized to the arrays. Of the total 20 400 oligonucleotides on the Pigoligoarray, 12 429 transcripts were putatively differentially expressed (DE). Analyses for tissue-specific expression [over-expressed in one tissue with respect to all the remaining three tissues (q < 0.01)] identified 958 DE transcripts in liver, 726 in muscle, 286 in uterine endothelium and 1027 in brain stem. These hybridization results were confirmed by quantitative PCR (QPCR) expression patterns for a subset of genes after affirming that cDNA and amplified antisense RNA (aRNA) exhibited similar QPCR results. Comparison to human ortholog expression confirmed the value of this array for experiments of both agricultural importance and for tests using pigs as a biomedical model for human disease.
Agid:
39257
Handle:
10113/39257