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Cellularity and enzymatic activity of adipose tissue in the Karagouniko dairy breed of sheep from birth to maturity

Rogdakis, E., Charismiadou, M., Orphanos, S., Panopoulou, E., Bizelis, I.
Journal of animal breeding and genetics 1997 v.114 no.1-6 pp. 385-396
adipocytes, adipose tissue, dairy sheep, enzyme activity, females, flocks, hyperplasia, hypertrophy, males, parturition, phosphogluconate dehydrogenase, sheep
SUMMARY: The cellular and enzymatic characteristics of dissected subcutaneous and perirenal adipose tissues were examined in a total of 102 male and female animals from commerical flocks of the Karagouniko dairy sheep. The animals were slaughtered in groups of three males and three females at 45-day intervals from birth to 720 days of age. The following determinations were made on each animal: (1) subcutaneous and perirenal chemical fat, (2) fat cell size and number and (3) the activities of NADP-isocitrate dehydrogenase and 6-phosphogluconate dehydrogenase in subcutaneous and perirenal adipose tissues. Subcutaneous adipose tissue expanded by a combination of fat cell hypertrophy and hyperplasia during the accelerating phase of the growth curve of chemical fat, after which the fat expansion was accomplished primarily by hyperplasia. The changes in perirenal chemical fat weight with increasing age were primarily due to hypertrophy of fat cells during the accelerating phase of the curve of chemical fat growth and due to a combination of hypertrophy and hyperplasia during the diminishing phase of the curve. The enzyme activities were higher in subcutaneous than in perirenal adipose tissue, with the NADP-isocitrate dehydrogenase being the most active enzyme in both tissues and sexes. The changes in enzyme activities in subcutaneous adipose tissue with increasing age were parallel to the changes in the rate of chemical fat weight during growth.