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Comparison of Laboratory Colonies and Field Populations of Tamarixia radiata, an Ectoparasitoid of the Asian Citrus Psyllid, Using Internal Transcribed Spacer and Cytochrome Oxidase Subunit I DNA Sequences

Barr, N.B., Hall, D.G., Weathersbee, A.A. III, Nguyen, R., Stansly, P., Qureshi, J.A., Flores, D.
Journal of economic entomology 2009 v.102 no.6 pp. 2325
insect pests, Diaphorina citri, plant pests, citrus fruits, greening disease, insect vectors, insect control, biological control, parasitoids, ectoparasites, Tamarixia, strains, genetic variation, insect genetics, genes, cytochrome-c oxidase, internal transcribed spacers, genetic markers, evolution, reproductive isolation, geographical variation, geographical distribution, biological control agents
The genetic diversity of Tamarixia radiata Waterston (Hymenoptera: Eulophidae) laboratory colonies derived from collections in China, northern Vietnam, Pakistan, and a mixed colony from Taiwan and southern Vietnam was evaluated using the internal transcribed spacer (ITS) region 1, ITS-2, and the 5′ end of the cytochrome oxidase subunit I gene. The strains share the same ITS sequence, consistent with the morphological hypothesis that the collections represent a single species. The COI marker was variable and could distinguish the northern Vietnam and Pakistan colonies from each other and from the other colonies. Comparison of COI sequences from field-collected populations of Puerto Rico, Guadeloupe, and Texas indicates that Florida is not a likely source of the introduction into Puerto Rico but is a likely source of the introduction into Texas.