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Molecular Identification of the Light Brown Apple Moth (Lepidoptera: Tortricidae) in California Using a Polymerase Chain Reaction Assay of the Internal Transcribed Spacer 2 Locus

Barr, N.B., Ledezma, L.A., Vasquez, J.D., Epstein, M., Kerr, P.H., Kinnee, S., Sage, O., Gilligan, T.M.
Journal of economic entomology 2009 v.102 no.6 pp. 2333
insect pests, moths, Epiphyas postvittana, plant pests, loci, insect control, etiology, pest identification, insect genetics, molecular genetics, genetic markers, internal transcribed spacers, polymerase chain reaction, gel electrophoresis, agarose, validity, population genetics, genetic variation, geographical distribution, California
A molecular protocol using a hemi-nested polymerase chain reaction (PCR) of the internal transcribed spacer region 2 (ITS2) is reported for the diagnosis of light brown apple moth, Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae), in California. This protocol distinguishes the light brown apple moth from other moths in California based on size differences of PCR amplicons that are visualized on agarose gels. The molecular diagnostic tool generated no false negatives based on analysis of 337 light brown apple moths collected from California, Hawaii, England, New Zealand, and Australia. Analysis of a data set including 424 moths representing other tortricid species generated correct identification for >95% of the samples and only two false positives. Of the 761 moths tested only fourteen produced no PCR amplicons and five generated inconclusive data.