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Enzymatic hydrolysis of cellulose hydrates
- Kobayashi, Kayoko, Kimura, Satoshi, Kim, Ung-Jin, Tokuyasu, Ken, Wada, Masahisa
- Cellulose 2012 v.19 no.3 pp. 967-974
- X-ray diffraction, adsorption, biodegradability, cellulose, endo-1,4-beta-glucanase, enzymatic hydrolysis, hydrolysis, hydrophobicity
- We prepared two cellulose hydrates, Na-cellulose IV and cellulose II hydrate, along with their respective anhydrous forms, cellulose II and IIâ², from microcrystalline cellulose. X-ray diffractometry analysis showed that the structure of the hydrophobic stacking sheet was conserved in the samples, but the distance between the sheets was in the order: cellulose II hydrateÂ >Â Na-cellulose IVÂ >Â cellulose II and IIâ². The hydrates exhibited an expanded structure compared with the anhydrous form from the incorporation of hydrate water, and cellulose II hydrate contained more hydrate water than Na-cellulose IV. Enzymatic hydrolysis of the samples was carried out at 37Â Â°C using solutions comprising a mixture of cellulase and Î²-glucosidase. The hydrates were hydrolyzed more efficiently than the anhydrous forms, and cellulose II hydrate showed a more efficient hydrolysis than Na-cellulose IV. This result also agrees well with the enzymatic adsorption properties of each sample, where the samples that adsorbed the greater amount of enzyme showed a higher degradability. The results obtained in this study provide useful knowledge on controlling the biodegradability of cellulose by converting its structure.