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Extraction and antioxidant property of polyhydroxylated naphthoquinone pigments from spines of purple sea urchin Strongylocentrotus nudus

Author:
Zhou, Da-Yong, Qin, Lei, Zhu, Bei-Wei, Wang, Xiao-Dong, Tan, Hui, Yang, Jing-Feng, Li, Dong-Mei, Dong, Xiu-Ping, Wu, Hai-Tao, Sun, Li-Ming, Li, Xiu-Ling, Murata, Yoshiyuki
Source:
Food chemistry 2011 v.129 no.4 pp. 1591-1597
ISSN:
0308-8146
Subject:
Echinoidea, adsorption, antioxidant activity, antioxidants, chelates, hybrids, lipid peroxidation, macrophages, pigments, resins, spectrometers, ultra-performance liquid chromatography
Abstract:
Purple sea urchin (Strongylocentrotus nudus) spines were dissolved in HCl–ethanol-aqueous solution. The polyhydroxylated 1,4-naphthoquinone (PHNQ) pigments were condensed and purified by using macroporous resin in static adsorption mode. PHNQ in the extract were characterised rapidly by using an ultra-performance liquid chromatography (UPLC) coupled to hybrid quadrupole orthogonal acceleration time-of-flight mass spectrometer (Q-TOFMS). Six known compounds including spinochrome E, 2,7-dihydroxynaphthazarin, spinochrome B, spinochrome C, spinochrome A and echinochrome A, and two new compounds with molecular formula of aminopentahydroxynaphthoquinone and acetylaminotrihydroxynaphthoquinone were identified tentatively. The pigment extract was evaluated for antioxidant activity by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity assay, Fe²⁺ chelating assay, reducing power assay, lipid peroxidation inhibition assay and tertiary-butyl hydroperoxide (t-BOOH)-induced macrophages protection assay. In all testing methods, the extract showed excellent activity, indicating the PHNQ from S. nudus spines are potential sources of natural antioxidants.
Agid:
398730