PubAg

"HOOF-Print’" genotyping and haplotype inference discriminates among Brucella spp. isolates from a small spatial scale

Author:

Almendra, Claudia, Silva, Teresa Luısa, Beja-Pereira, Albano, Ferreira, Ana Cristina, Ferrao-Beck, Lenia, Corrêa de Sá, Maria Inácia, Bricker, Betsy J., Luikart, Gordon

Source:

Infection, genetics, and evolution 2009 v.9 no.1 pp. 104

Subject:

Brucella, animal pathogenic bacteria, Brucella melitensis, genotype, haplotypes, microbial genetics, genetic techniques and protocols

Abstract:

We demonstrate that the "HOOF-Print" assay provides high power to discriminate among Brucella isolates collected on a small spatial scale (within Portugal). Additionally, we illustrate how haplotype identification using non-random association among markers allows resolution of B. melitensis biovars (1 and 3). We recommend that future studies use haplotype identification when analyzing multilocus population genetic data to help discriminate among microbial isolates such as Brucella.

Agid:

40072

Handle:

10113/40072

http://dx.doi.org/10.1016/j.meegid.2008.10.007