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Genetic characterization and mapping of major gene resistance to potato leafroll virus in Solanum tuberosum ssp. andigena

Velásquez, A. C., Mihovilovich, E., Bonierbale, M.
Theoretical and applied genetics 2007 v.114 no.6 pp. 1051-1058
Potato leafroll virus, Solanum tuberosum, amplified fragment length polymorphism, chromosomes, dihaploidy, landraces, linkage groups, major genes, marker-assisted selection, microsatellite repeats, resistance mechanisms, tetraploidy, viruses
Major gene inheritance of resistance to Potato leafroll virus (PLRV) was demonstrated in a parthenogenic population derived from the highly resistant tetraploid andigena landrace, LOP-868. This major gene or chromosome region seems to control a single mechanism for resistance to infection and virus accumulation in this source. About 149 dihaploid lines segregated in a ratio of 107 resistant to 32 susceptible, fitting the expected ratio for inheritance of a duplex gene under random chromatid segregation. A tetraploid AFLP map was constructed using as reference the ultra high density (UHD) map. All AFLP markers associated with PLRV resistance mapped to the same linkage group. Map position was confirmed by analysis of previously-mapped SSR markers. Rl adg is located on the upper arm of chromosome V, at 1 cM from its most closely linked AFLP marker, E35M48.192. This marker will be used to develop allele-specific primers or a pair of flanking PCR-based markers for their use in marker assisted selection.