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Sox9 Is Upstream of MicroRNA-140 in Cartilage

Author:
Nakamura, Yukio, He, Xinjun, Kato, Hiroyuki, Wakitani, Shigeyuki, Kobayashi, Tatsuya, Watanabe, Sumiko, Iida, Atsumi, Tahara, Hideaki, Warman, Matthew L., Watanapokasin, Ramida, Postlethwait, John H.
Source:
Applied biochemistry and biotechnology 2012 v.166 no.1 pp. 64-71
ISSN:
0273-2289
Subject:
Danio rerio, cartilage, chondrocytes, humans, mutants, platelet-derived growth factor, receptors, small interfering RNA, transcription (genetics)
Abstract:
MicroRNA-140 (miR-140) is specifically expressed in developing cartilage tissues. We have previously reported that miR-140 plays an important role during palatal cartilage development by modulating platelet-derived growth factor receptor alpha (pdgfra) in zebrafish. However, the regulatory mechanism of miR-140 in cartilage is still unknown. Using developing zebrafish, sox9a mutant (sox9a−/−) and sox9b mutant (sox9b−/−) zebrafish and SOX9 small interfering RNA in human chondrocytes, T/C-28 cells, we found that miR-140 is regulated by the cartilage master transcription regulator Sox9 in zebrafish and mammalian cells.
Agid:
402749