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Molecular characterization of artemisia virus A, a new sobemovirus isolated from Artemisia annua

Author:
Ramel, Maria-Elena, Gugerli, Paul, Besse, Sébastien, Simonnet, Xavier, Balmelli, Carole
Source:
Archives of virology 2013 v.158 no.2 pp. 463-466
ISSN:
0304-8608
Subject:
Artemisia annua, RNA, Ryegrass mottle virus, electron microscopy, genome, leaf extracts, nomen novum, open reading frames, phylogeny, polluted soils, roots, soil sampling, virion, viruses, Switzerland
Abstract:
A distinct sobemovirus was isolated from diseased Artemisia annua plants grown in experimental culture plots in Switzerland. Electron microscopy performed on extracts of leaf and root samples of a diseased A. annua plant revealed icosahedral-30 nm viral particles. The complete nucleotide sequence of the viral genome was determined. The single positive-strand RNA of 4138 nt encodes four open reading frames with an organization similar to that described for sobemoviruses. Phylogenetic analysis revealed a close relationship to ryegrass mottle virus. The virus was efficiently acquired by healthy A. annua from contaminated soil samples. “Artemisia virus A” is tentatively proposed as a name for this new candidate member of the unassigned genus Sobemovirus.
Agid:
407357