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Physiological characterization of human muscle acetylcholine receptors from ALS patients

Author:
Palma, Eleonora, Inghilleri, Maurizio, Conti, Luca, Deflorio, Cristina, Frasca, Vittorio, Manteca, Alessia, Pichiorri, Floriana, Roseti, Cristina, Torchia, Gregorio, Limatola, Cristina, Grassi, Francesca, Miledi, Ricardo
Source:
Proceedings of the National Academy of Sciences of the United States of America 2011 v.108 no.50 pp. 20184-20188
ISSN:
0027-8424
Subject:
Xenopus, acetylcholine, biopsy, cholinergic receptors, humans, mice, models, motor neurons, nicotine, oocytes, paralysis, pathogenesis, patients, sclerosis
Abstract:
Amyotrophic lateral sclerosis (ALS) is characterized by progressive degeneration of motor neurons leading to muscle paralysis. Research in transgenic mice suggests that the muscle actively contributes to the disease onset, but such studies are difficult to pursue in humans and in vitro models would represent a good starting point. In this work we show that tiny amounts of muscle from ALS or from control denervated muscle, obtained by needle biopsy, are amenable to functional characterization by two different technical approaches: "microtransplantation" of muscle membranes into Xenopus oocytes and culture of myogenic satellite cells. Acetylcholine (ACh)-evoked currents and unitary events were characterized in oocytes and multinucleated myotubes. We found that ALS acetylcholine receptors (AChRs) retain their native physiological characteristics, being activated by ACh and nicotine and blocked by α-bungarotoxin (α-BuTX), d-tubocurarine (dTC), and galantamine. The reversal potential of ACh-evoked currents and the unitary channel behavior were also typical of normal muscle AChRs. Interestingly, in oocytes injected with muscle membranes derived from ALS patients, the AChRs showed a significant decrease in ACh affinity, compared with denervated controls. Finally, riluzole, the only drug currently used against ALS, reduced, in a dose-dependent manner, the ACh-evoked currents, indicating that its action remains to be fully characterized. The two methods described here will be important tools for elucidating the role of muscle in ALS pathogenesis and for developing drugs to counter the effects of this disease.
Agid:
407423