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Development of a Monoclonal Antibody-Based Immunochemical Assay for Liquiritin and Its Application to the Quality Control of Licorice Products

Fujii, Shunsuke, Morinaga, Osamu, Uto, Takuhiro, Nomura, Shuichi, Shoyama, Yukihiro
Journal of agricultural and food chemistry 2014 v.62 no.15 pp. 3377-3383
antigens, dietary supplements, enzyme-linked immunosorbent assay, hemocyanin, high performance liquid chromatography, hybridomas, immunochemistry, licorice, liqueurs, mice, monoclonal antibodies, quality control, roots, sweets
Liquiritin was reacted with a keyhole limpet hemocyanin (KLH) to synthesize a liquiritin-KLH conjugate as an immunogen for mice. A hybridoma cell line named 2F8 secreted a monoclonal antibody (mAb) against liquiritin, which was applied to an enzyme-linked immunosorbent assay (ELISA) for liquiritin. ELISA showed a good linear range from 0.39 to 25 mu g/mL of liquiritin. The maximum relative standard deviation (RSD) values for the intra-assay and interassay were approximately 5%. The recovery rates of liquiritin were in the range of 100.9-103.7%, and the concentrations of liquiritin in various licorice roots, as determined by ELISA, showed a good correlation with those analyzed by high-performance liquid chromatography (HPLC; R(2) = 0.948). These results suggested that ELISA with anti-liquiritin mAb could be a simple, rapid, convenient, and accurate method for the high-throughput analysis of liquiritin in various licorice products including liqueurs, sweets, and food supplements.