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Antimicrobial and inhibition on heat-induced protein denaturation of constituents isolated from Polygonatumverticillatum rhizomes

Khan, Haroon, Saeed, Muhammad, Rauf, Abdur, Khan, Muhammad Atif, Muhammad, Naveed
Natural product research 2015 v.29 no.22 pp. 2160-2163
Aspergillus flavus, Aspergillus niger, Bacillus cereus, Bacillus subtilis, Escherichia coli, Fusarium oxysporum, Gram-negative bacteria, Polygonatum, Salmonella Typhi, Staphylococcus aureus, Staphylococcus epidermidis, Trichoderma harzianum, anti-infective agents, denaturation, diosgenin, fungi, rhizomes
This study was designed to assess the susceptibility of various microorganisms and inhibition on heat-induced protein denaturation against diosgenin and santonin, isolated from Polygonatum verticillatum rhizomes. Both diosgenin and santonin showed significant zone of inhibition when studied against various Gram-positive (Bacillus subtilis , Bacillus cereus , Staphylococcus aureus and Staphylococcus epidermidis) and Gram-negative bacteria (Escherichia coli and Salmonella typhi). In antifungal assay, only santonin exhibited profound sensitivity against various fungi (Aspergillus flavus , Aspergillus niger , Trichoderma harzianum and Fusarium oxysporum) used in the test. Both diosgenin and santonin also exhibited marked attenuation on heat-induced protein denaturation in a concentration-dependent manner with EC ₅₀ values of 375 and 310 μg/mL, respectively. In conclusion, both the isolated compounds have antimicrobial potential supported by strong inhibition on protein denaturation and thus support the antimicrobial uses of plant in traditional system of treatment.