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Determination of the hydroxycinnamate profile of 12 members of the Asteraceae family

Jaiswal, Rakesh, Kiprotich, Joseph, Kuhnert, Nikolai
Phytochemistry 2011 v.72 no.8 pp. 781-790
Achillea millefolium, Arnica montana, Centaurea benedicta, Cichorium intybus, Cynara cardunculus, Echinops, Inula helenium, Lactuca sativa, Petasites hybridus, Solidago virgaurea, Tanacetum parthenium, chlorogenic acid
The hydroxycinnamates of the leaves of 12 plants of the Astreraceae family, Achillea millefolium, Arnica montana, Artemesia dracunculus, Cichorium intybus, Cnicus benedictus, Cynara scolymus, Echinops humilis, Inula helenium, Lactuca sativa, Petasites hybridus, Solidago virgaurea, and Tanacetum parthenium were investigated qualitatively by LC–MSⁿ. Thirty-nine chlorogenic acids were detected and all characterized to regioisomeric level on the basis of their fragmentation pattern in the tandem MS spectra, most of them for the first time from these sources with two of them previously not reported in nature. Both chlorogenic acids based on trans and cis-cinnamic acid substituents were identified. Assignment to the level of individual regioisomers was possible for seven caffeoylquinic acids (1–7), 11 dicaffeoylquinic acids (17–27), six feruloylquinic acids (9–14), two p-coumaroylquinic acids (15–16), two caffeoyl–feruloylquinic acids (28 and 29), four caffeoyl-p-coumaroylquinic acids (30–33), three dicaffeoyl–succinoylquinic acids (34–36), two dicaffeoyl–methoxyoxaloylquinic acids (37 and 38), and one tricaffeoylquinic acid (39). Furthermore, one caffeoylshikimic acid (40), one caffeoyltartaric acid (41), three dicaffeoyltartaric acids (42–44), and three caffeoyl–feruloyltartaric acids (45–47) were detected and shown to possess characteristic tandem MS spectra and were tentatively assigned on the basis of their retention time and previously developed hierarchical keys.