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Selective microbial aerosolization in biogas demonstrated by quantitative PCR

Author:
Moletta-Denat, Marina, Bru-Adan, Valérie, Delgenes, Jean-Philippe, Hamelin, Jérôme, Wéry, Nathalie, Godon, Jean-Jacques
Source:
Bioresource technology 2010 v.101 no.19 pp. 7252-7257
ISSN:
0960-8524
Subject:
Archaea, DNA, Propionibacterium acnes, Staphylococcus, Synergistes, air, anaerobic digesters, bacteria, biogas, natural gas, pathogens, polymerase chain reaction, sludge
Abstract:
Aerosolization of Bacteria, Archaea, Synergistes, Staphylococcus spp. and Propionibacterium acnes was investigated in situ with quantitative real-time PCR of DNA isolated from sludge and biogases of anaerobic digesters. The data revealed that in biogas, Staphylococcus spp. and P. acnes were, respectively, aerosolized 30 and 220 times more and Archaea and Synergistes, respectively, 8 and 20 times less aerosolized than Bacteria. This is the first demonstration of selective microbial aerosolization for anaerobic digestors microorganisms. This study illustrates the fact that some microbial groups, such as opportunistic pathogens, are more susceptible to be aerosolized, since they use air as a dissemination vector, and that this has to be taken in account when up-grading biogas into natural gas networks.
Agid:
411152