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A defensin-like peptide from Phaseolus vulgaris cv. ‘King Pole Bean’
- Wong, Jack H., Ip, David C.W., Ng, T.B., Chan, Y.S., Fang, F., Pan, W.L.
- Food chemistry 2012 v.135 no.2 pp. 408-414
- Candida albicans, Mycosphaerella, Phaseolus vulgaris, Saccharomyces cerevisiae, agarose, anion exchange, chromatography, dyes, filtration, green beans, membrane permeability, microbial growth, sequence homology
- A 5447Da antifungal peptide with an N-terminal sequence highly homologous to plant defensins was purified from Phaseolus vulgaris cv. ‘King Pole Bean’ by anion-exchange chromatography on Q Sepharose and FPLC-gel filtration on Superdex 75. The isolated peptide inhibited growth of a number of fungal species, including Mycosphaerella arachidicola, Saccharomyces cerevisiae and Candida albicans, with IC₅₀ values of 3.9, 4.0 and 8.4μM, respectively. Using the membrane non-permeable DNA-binding dye SYTOX green, it was found that the peptide increased the cell membrane permeability of M. arachidicola, S. cerevisiae and C. albicans.