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Novel extracellular hyper acidophil and thermostable Î±âamylase from Micrococcus sp.NS 211
- Samie, Nima, Reddy, Padma R. M., Ashouri, Masoumeh
- DieStaÌrke = 2012 v.64 no.2 pp. 136-144
- EDTA (chelating agent), Micrococcus, bacteria, calcium chloride, chromatography, detergents, enzyme activity, genes, laundry, magnesium chloride, molecular weight, pH, sodium chloride, sodium citrate, starch, temperature, thermal stability, urea
- Among several bacteria examined in this study, a hyper acidophil and thermostable Micrococcus sp.NS 211 designated as M.Amy NS 211 was selected for production of amylase using starch agar plates with following incubation at 85Â°C. Identification by 16SrRNA on selected bacterium disclosed the highest similarity for protean regions of this gene, 27 F and 1492R as Micrococcus sp.NS 211. Although activity of M.Amy NS 211 was established at temperatures between 70 and 110Â°C and pH ranges 1.2â8.0, the optimum temperature and pH was achieved at 85Â°C and 3.5 in sodium citrate buffer system respectively. Twoâstep chromatography was performed using (CM BioâGel A) and (BioâGel Aâ150) columns to purify 84âkDa hyper acidophil and thermostable Î±âamylase. SDSâPAGE analysis showed molecular mass and amylolytic activity as single band. Enhancement of enzyme activity was obtained in presence of 5âmM MnCl2 (298%), CaCl2 (347%), FeCl2 (211%), MgCl2 (253%), ZnCl2 (146%), NiCl (142%), NaCl (141%), Naâsulfate (153%) while inhibition was observed with (5âmM) EDTA, PMSF (3âmM), urea (8âM), and SDS (1%) at 143, 134, 43, and 119%, respectively. M.Amy NS 211 can be applied in laundry detergents, textile, and modern relevant industrial processes at extreme temperatures and under acidic conditions.