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Application of a highly sensitive magnetic solid phase extraction for phytochemical compounds in medicinal plant and biological fluids by ultra‐high performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry

Cao, Wan, Yi, Ling, Ye, Li‐Hong, Cao, Jun, Hu, Shuai‐Shuai, Xu, Jing‐Jing, Peng, Li‐Qing, Zhu, Qiong‐Yao, Zhang, Qian‐Yun
Electrophoresis 2015 v.36 no.19 pp. 2404-2412
adsorption, correlation, electrophoresis, emodin, graphene oxide, iron oxides, medicinal plants, nanoparticles, rats, rhubarb, solid phase extraction, tandem mass spectrometry, ultra-performance liquid chromatography, urine
A highly sensitive method using reduced graphene oxide with iron oxide (rGO/Fe₃O₄) as the sorbent in magnetic SPE has been developed for the purification of five anthraquinones (emodin, rhein, aloeemodin, physcion, and chrysophanol) in rhubarb and rat urine by ultra‐HPLC coupled with quadrupole TOF/MS. The extraction was accomplished by adding trace amount rGO/Fe₃O₄ suspension to 200 mL of aqueous mixture, and the excellent adsorption capacity of the nanoparticles was fully demonstrated in this procedure. Under the optimized conditions, the calibration curves were linear in the concentration range of 0.05–27.77 ng/mL with correlation coefficients varying from 0.9902 to 0.9978. The LODs ranged from 0.28 to 58.99 pg/mL. The experimental results indicated that the proposed method was feasible for the analysis of anthraquinones in rhubarb and urine samples.