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Capillary electrophoretic studies on quantum dots and histidine appended peptides self‐assembly
- Wang, Jianhao, Li, Jingyan, Chen, Yao, Teng, Yiwan, Wang, Cheli, Li, Jinchen, Liu, Li, Dong, Bingyu, Qiu, Lin, Jiang, Pengju
- Electrophoresis 2015 v.36 no.19 pp. 2419-2424
- electrophoresis, fluorescence, histidine, ligands, mixing, nanoparticles, peptides, quantum dots
- Herein, we designed four peptides appended with different numbers of histidine (Hisₙ‐peptide). We launched a systematic investigation on quantum dots (QDs) and Hisₙ‐peptide self‐assembly in solution using fluorescence coupled CE (CE‐FL). The results indicated that CE‐FL was a powerful method to probe how ligands interaction on the surface of nanoparticles. The self‐assembly of QDs and peptide was determined by the numbers of histidine. We also observed that longer polyhistidine tags (n ≤ 6) could improve the self‐assembly efficiency. Furthermore, the formation and separation of QD‐peptide assembly were also studied by CE‐FL inside a capillary. The total time for the mixing, self‐assembly, separation, and detection was less than 10 min. Our method greatly expands the application of CE‐FL in QDs‐based biolabeling and bioanalysis.