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Effect of additives on mesophilic α-amylase and its application in the desizing of cotton fabrics

Fu, Kang-li, Wang, Ding-bang, Li, Yang, Lu, Da-nian
The journal of the Textile Institute 2015 v.106 no.12 pp. 1322-1327
acetates, additives, alpha-amylase, calcium, catalytic activity, chitosan, cotton fabric, histidine, sodium, temperature, thermal stability
An investigation was carried out on thermal stability of α-amylase. The influence of various additives (calcium acetate, sodium lactate, L-histidine, and water-soluble chitosan) on the stability of α-amylase was studied. Results showed the inactivation behavior of α-amylase with or without additives all followed the first-order kinetics. All additives (Ca2+, sodium lactate, L-histidine, and water-soluble chitosan) displayed good stabilizing effect on α-amylase lower than 80 °C, and only water-soluble chitosan had an efficient stabilizing effect on α-amylase when the treatment temperature exceeds 80 °C. All additives improved the catalytic activity of α-amylase at 70–90 °C, and the appearance of water-soluble chitosan increased the catalytic activity of α-amylase at 90 °C sharply. A desizing ratio of 68.42% was obtained by treating the cotton fabrics in the buffer solution at 100 °C without α-amylase. To obtain a desizing ratio exceed 95% when fabrics were treated at 100 °C for 10 min, the addition of water-soluble chitosan saves 2/3 α-amylase dosage. Moreover, water-soluble chitosan showed a further improvement in desizing effect than the additive of calcium acetate.