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Isolation of a Paenibacillus sp. Strain and Structural Elucidation of Its Broad-Spectrum Lipopeptide Antibiotic

Author:
Guo, Yaoqi, Huang, En, Yuan, Chunhua, Zhang, Liwen, Yousef, Ahmed E.
Source:
Applied and environmental microbiology 2012 v.78 no.9 pp. 3156-3165
ISSN:
0099-2240
Subject:
Escherichia coli O157, Gram-positive bacteria, Listeria monocytogenes, Paenibacillus, Salmonella enterica subsp. enterica serovar Typhimurium, Staphylococcus aureus, amidase, amino acids, antibiotics, antimicrobial properties, esters, genetic techniques and protocols, heat stability, liquid chromatography, mass spectrometry, medical foods, nuclear magnetic resonance spectroscopy, pH, triacylglycerol lipase
Abstract:
This research was initiated to search for novel antimicrobial compounds produced by food or environmental microorganisms. A new bacterial strain, designated OSY-SE, which produces a unique and potent antimicrobial agent was isolated from soil. The isolate was identified as a Paenibacillus sp. through cultural, biochemical, and genetic analyses. An antimicrobial compound was extracted from Paenibacillus OSY-SE with acetonitrile and purified using liquid chromatography. After analyses by mass spectrometry (MS) and nuclear magnetic resonance (NMR), the antimicrobial compound was determined to be a cyclic lipopeptide consisting of a C15 fatty acyl (FA) chain and 13 amino acids. The deduced sequence is FA-Orn-Val-Thr-Orn-Ser-Val-Lys-Ser-Ile-Pro-Val-Lys-Ile. The carboxyl-terminal Ile is connected to Thr by ester linkage. The new compound, designated paenibacterin, showed antagonistic activities against most Gram-positive and Gram-negative bacteria tested, including Listeria monocytogenes, methicillin-resistant Staphylococcus aureus, Escherichia coli O157:H7, and Salmonella enterica serovar Typhimurium. Paenibacterin is resistant to trypsin, lipase, α-glucosidase, and lysozyme. Its antimicrobial activity was lost after digestion by pronase and polymyxin acylase. Paenibacterin is readily soluble in water and fairly stable to exposure to heat and a wide range of pH values. The new isolate and its antimicrobial agent are being investigated for usefulness in food and medical applications.
Agid:
419904