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Fine mapping of a gene for low-tiller number, Ltn, in japonica rice (Oryza sativa L.) variety Aikawa 1

Author:
Fujita, Daisuke, Ebron, Leodegario A., Araki, Etsuko, Kato, Hiroshi, Khush, Gurdev S., Sheehy, John E., Lafarge, Tanguy, Fukuta, Yoshimichi, Kobayashi, Nobuya
Source:
Theoretical and applied genetics 2010 v.120 no.6 pp. 1233-1240
ISSN:
0040-5752
Subject:
Oryza sativa, agronomic traits, chromosome mapping, chromosomes, crop production, crossing, genes, genetic markers, marker-assisted selection, microsatellite repeats, rice, tillering
Abstract:
Tillering is one of the most important agronomic traits related to grain production in rice (Oryza sativa L.). A japonica-type variety, Aikawa 1, is known to have low-tiller number. The detailed location of a low-tillering gene, Ltn, which has been localized on chromosome 8 in Aikawa 1, was confirmed by molecular mapping. Using BC₅F₂ individuals derived from a cross between IR64 and Aikawa 1, the low-tillering gene was mapped to an interval defined by SSR markers ssr5816-3 and A4765. This was designated as Ltn because there was no reported gene for tillering in the region of chromosome 8. Through high-resolution linkage analysis, the candidate region of Ltn was located between DNA markers ssr6049-23 and ind6049-1 corresponding to 38.6 kbp on the Nipponbare genome sequence. These DNA markers, which were tightly linked to Ltn, are useful for marker-assisted selection in breeding studies.
Agid:
420319