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Alteromonas halophila sp. nov., a new moderately halophilic bacterium isolated from a sea anemone

Author:
Chen, Yi-Guang, Xiao, Huai-Dong, Tang, Shu-Kun, Zhang, Yu-Qin, Borrathybay, Entomack, Cui, Xiao-Long, Li, Wen-Jun, Liu, Yan-Qi
Source:
Antonie van Leeuwenhoek 2009 v.96 no.3 pp. 259-266
ISSN:
0003-6072
Subject:
Alteromonas, Anthozoa, DNA, Formicidae, bacteria, fatty acids, islands, new species, nucleotide sequences, pH, phospholipids, phylogeny, ribosomal RNA, salts, South China Sea
Abstract:
A pale yellow-colored, moderately halophilic, Gram-negative, catalase- and oxidase-positive, non-sporulating, rod-shaped, motile, aerobic bacterium, designated strain JSM 073008T, was isolated from a sea anemone (Anthopleura xanthogrammica) collected from Naozhou Island, Leizhou Bay, South China Sea. The organism was able to grow with 1-20% (w/v) total salts (optimum, 5-10%), at pH 6.0-10.0 (optimum, pH 7.5) and 10-40°C (optimum, 25-30°C). The major cellular fatty acids were C₁₆:₀, C₁₆:₁ ω7c/iso-C₁₅:₀ 2-OH and C₁₈:₁ ω7c. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid. The predominant respiratory quinone was Q-8 and the genomic DNA G + C content was 47.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 073008T should be assigned to the genus Alteromonas, being most closely related to Alteromonas hispanica F-32T (sequence similarity 96.9%), followed by Alteromonas genovensis LMG 24078T (96.6%) and Alteromonas litorea TF-22T (96.4%). The sequence similarities between the novel isolate and the type strains of other recognized Alteromonas species ranged from 95.9% (with Alteromonas stellipolaris ANT 69aT) to 94.5% (with Alteromonas simiduii BCRC 17572T). The combination of phylogenetic analysis, phenotypic characteristics and chemotaxonomic data supported the view that strain JSM 073008T represents a new species of the genus Alteromonas, for which the name Alteromonas halophila sp. nov. is proposed. The type strain is JSM 073008T (=CCTCC AA 207035T = KCTC 22164T).
Agid:
422481