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Spectrophotometrically assayed inhibitory effects of mercuric compounds on anabaena flos‐aquae and anacystis nidulans (cyanophyceae)1

Thomas, Dempsey L., Mantes, Joseph G.
Journal of phycology 1978 v.14 no.4 pp. 494-499
Algae, Anabaena flos-aquae, absorbance, acetates, bioassays, bleaching, mercuric chloride, mercury, nutrient content, organisms
The growth‐related inhibitory effects of mercuric chloride (MC), methylmercuric chloride (MMC) and phenylmercuric acetate (PMA) (each at 1, 10, 10,² 10³ ppb) were measured in Anabaena flos‐aquae (Lyng.) Bréb. and Anacystis nidulans (Richt.) Drouet & Daily. Optical density changes of control cultures compared against those of experimental cultures showed that MC was the least inhibitory of the compounds. MMC. was the most inhibitory, producing statistically significant inhibition at a concentration as low as I ppb in Anabaena. PMA was more inhibitory than MC but less than MMC. Effects caused by the mercury compounds included bleaching of individual cells, cell size changes and destruction of whole cells; the degree and extent of these effects depended on the compound and its concentration in the nutrient medium. The high sensitivities of the algae tested suggested the possibility of using them as test organisms in bioassays for mercury.