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A new cell line from Spodoptera exigua (Lepidoptera: Noctuidae) and its differentially expressed genes
- Zhang, A., Li, X., Zhang, H., Wang, H., Miao, L., Zhang, J., Qin, Q.
- Journal of applied entomology 2012 v.136 no.8 pp. 632-637
- Autographa californica, Helicoverpa armigera, Hyphantria cunea, Nucleopolyhedrovirus, RNA, Spodoptera exigua, cattle, gene expression, genes, ovaries, polymerase chain reaction, protein kinase C, proteins, receptors
- A new cell line, designated IOZCASâSpex XI, was established from the pupal ovaries of Spodoptera exigua (Lepidoptera: Noctuidae) in TNMâFH medium containing 10% foetal bovine serum. The spherical cells were predominant among the various cell types. The populationâdoubling time during the logarithmic phase of growth was 81.7âh. It was confirmed that the cell line originated from S.Â exigua by DAFâPCR technique. Analysis of susceptibility to baculovirus showed that the new cell line was susceptible to S.Â exigua nucleopolyhedrovirus (SeNPV), Autographa californica multiple NPV (AcMNPV) and slightly susceptible to S.Â litura NPV (SpltNPV), while not permissive to Helicoverpa armigera NPV and Hyphantria cunea NPV (HcNPV). RealâTime PCR analysis was carried out to compare some differentially expressed genes between the cell line and the primary culture. The result showed that marked significant differences were observed in the expression of the genes of SUMOâ1 activating enzyme, BCCIPâlike protein, 10âkDa HSP, CypA, receptor for activated PKC, PDIâlike protein ERp57, ALDH, DEAD box ATPâdependent RNA helicaseâlike protein (Pâ<â0.01), while a significant difference was obtained in the expression of GST gene between the cell line and the primary culture (Pâ<â0.05).