Main content area

A new cell line from Spodoptera exigua (Lepidoptera: Noctuidae) and its differentially expressed genes

Zhang, A., Li, X., Zhang, H., Wang, H., Miao, L., Zhang, J., Qin, Q.
Journal of applied entomology 2012 v.136 no.8 pp. 632-637
Autographa californica, Helicoverpa armigera, Hyphantria cunea, Nucleopolyhedrovirus, RNA, Spodoptera exigua, cattle, gene expression, genes, ovaries, polymerase chain reaction, protein kinase C, proteins, receptors
A new cell line, designated IOZCAS‐Spex XI, was established from the pupal ovaries of Spodoptera exigua (Lepidoptera: Noctuidae) in TNM‐FH medium containing 10% foetal bovine serum. The spherical cells were predominant among the various cell types. The population‐doubling time during the logarithmic phase of growth was 81.7 h. It was confirmed that the cell line originated from S. exigua by DAF‐PCR technique. Analysis of susceptibility to baculovirus showed that the new cell line was susceptible to S. exigua nucleopolyhedrovirus (SeNPV), Autographa californica multiple NPV (AcMNPV) and slightly susceptible to S. litura NPV (SpltNPV), while not permissive to Helicoverpa armigera NPV and Hyphantria cunea NPV (HcNPV). Real‐Time PCR analysis was carried out to compare some differentially expressed genes between the cell line and the primary culture. The result showed that marked significant differences were observed in the expression of the genes of SUMO‐1 activating enzyme, BCCIP‐like protein, 10 kDa HSP, CypA, receptor for activated PKC, PDI‐like protein ERp57, ALDH, DEAD box ATP‐dependent RNA helicase‐like protein (P < 0.01), while a significant difference was obtained in the expression of GST gene between the cell line and the primary culture (P < 0.05).