PubAg

Main content area

(-)-Gossypol Reduces Invasiveness in Metastatic Prostate Cancer Cells

Author:
Huang, Yi-Wen, Wang, Li-Shu, Dowd, Michael K., Wan, Peter J., Lin, Young C.
Source:
Anticancer research 2009 v.29 no.6 pp. 2179
Subject:
gossypol, prostatic neoplasms, chemical constituents of plants, metastasis, cottonseed, anticarcinogenic activity, apoptosis, animal disease models, rats, dose response, animal proteins, cell lines, cell growth
Abstract:
Background: Acquisition of metastatic ability by prostatic cancer cells is the most lethal aspect of prostatic cancer progression. (-)-Gossypol, a polyphenolic compound present in cottonseeds, possesses anti-proliferative and proapoptotic effects in various cancer cells. Materials and Methods: In this study, the differences between MAT-LyLu, rat prostate cancer cells, with a novel isolated subline from metastasized tumors in the lungs of MAT-LyLu-bearing Copenhagen rats (MLL cells) were compared with respect to cell growth and invasion. The effects of (-)-gossypol on cell viability, colony formation, invasive ability and cell migration in MAT-LyLu and MLL cells were also evaluated. Results: Results showed that MLL cells displayed higher growth ability, colony formation and aggressive penetration than those of MAT-LyLu cells. MLL cells possess lower protein expression of Bcl-xL and nm23-H1 than those of MAT-LyLu cells, implying differences in invasive ability. Moreover, (-)-gossypol treatment induced a dose-dependent inhibition of invasive activity and cell viability and reduced Bcl-2 and Bcl-xL proteins but induced nm23-H1 protein in both cell lines. Conclusion: These findings illustrated that (-)-gossypol reduced in vitro invasion of both the parental MAT-LyLu cells and the isolated MLL cells, suggesting that (-)-gossypol might serve as a chemotherapeutic and/or chemopreventive agent.
Agid:
42619
Handle:
10113/42619