PubAg

Main content area

Antiinflammatory activities of flavonoids and a triterpene caffeate isolated from Bauhinia variegata

Author:
Rao, Yerra Koteswara, Fang, Shih‐Hua, Tzeng, Yew‐Min
Source:
Phytotherapy research 2008 v.22 no.7 pp. 957-962
ISSN:
0951-418X
Subject:
Bauhinia variegata, acids, aerial parts, anti-inflammatory activity, anti-inflammatory agents, ethers, hesperidin, interferons, interleukins, isorhamnetin, kaempferol, lipopolysaccharides, macrophages, nitric oxide, tumor necrosis factors
Abstract:
In the continuing search for novel antiinflammatory agents, six flavonoids, namely kaempferol (1), ombuin (2), kaempferol 7,4′‐dimethyl ether 3‐O‐β‐d‐glucopyranoside (3), kaempferol 3‐O‐β‐d‐glucopyranoside (4), isorhamnetin 3‐O‐β‐d‐glucopyranoside (5) and hesperidin (6), together with one triterpene caffeate, 3β‐trans‐(3,4‐dihydroxycinnamoyloxy)olean‐12‐en‐28‐oic acid (7) were isolated from the non‐woody aerial parts of Bauhinia variegata. Compounds 1–7 were evaluated as inhibitors of some macrophage functions involved in the inflammatory process. These seven compounds significantly and dose dependently inhibited lipopolysaccharide (LPS) and interferon (IFN)‐γ induced nitric oxide (NO), and cytokines [tumor necrosis factor (TNF)‐α and interleukin (IL)‐12]. The concentration causing a 50% inhibition (IC₅₀) of NO, TNF‐α and IL‐12 production by compounds 1, 2 and 7 was approximately 30, 50 and 10 µM, respectively, while at 50, 200 and 40 µM compounds 3, 4, and 5, 6 showed 15–30% inhibition, respectively. On the other hand, compounds 3 and 7 showed no inhibitory effect, while compounds 1, 4–6 reduced by around 10–30% the synthesis of NO by macrophages, when inducible NO synthase was already expressed with LPS/IFN‐γ for 24 h. These experimental findings lend pharmacological support to the suggested folkloric uses of the plant B. variegata in the management of inflammatory conditions. Copyright © 2008 John Wiley & Sons, Ltd.
Agid:
427505